Porcine lipopolysaccharide binding protein (LBP) ELISA kit (3.13-200 ng/mL)

Cat. No.: 0126WXX-770

Assay Type: Quantitative sandwich ELISA

Target Species: Porcine

Assay Target: LBP

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Porcine lipopolysaccharide binding protein (LBP) ELISA kit (3.13-200 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Porcine lipopolysaccharide binding protein (LBP) ELISA kit (3.13-200 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LBP in porcine samples with a range of 3.13-200 ng/mL and a minimum detectable dose (sensitivity) of 1.88 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with an antibody specific to porcine LBP. Standards or samples are added to the wells to bind with the capture antibody. Subsequently, a biotinylated detection antibody for porcine LBP and an avidin-HRP conjugate are added successively. After washing away free components, a substrate solution is added to develop a blue color, which turns yellow upon adding the stop solution. The O.D. value, measured at 450 nm ± 2 nm, is proportional to the concentration of Porcine LBP.

Background: LBP is a secreted protein primarily present in the extracellular space. Its core function is to bind bacterial lipopolysaccharide (LPS), a glycolipid found on the outer membrane of all Gram-negative bacteria. LBP is significant in obesity research because it plays a pivotal role in the link between inflammation, gut microbiota, and metabolic health. By studying LBP's mechanisms of action and its level changes, scientists can better understand how obesity leads to systemic inflammation and insulin resistance, potentially developing therapies targeting LBP or its associated signaling pathways.

Synonyms: LPS-binding protein

Applications: Porcine lipopolysaccharide binding protein (LBP) ELISA kit (3.13-200 ng/mL) is used to quantify LBP in serum, plasma, and other biological fluids of porcine, providing data to support research in a wide range of areas, including Infection immunity, obesity, etc.

Research Area: Infection immunity; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 3.13-200 ng/mL

Sensitivity: 1.88 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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