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Vitamin B12 test kit

Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-1262
Assay Type: Quantitative competitive ELISA
Target Species: Mouse
Assay Target: PFKFB3
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Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) ELISA kit-Quantitative competitive
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Product Overview

Description: Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) ELISA kit-Quantitative competitive is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PFKFB3 in mouse samples with a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.
Assay Principle: The ELISA analytical biochemical technique is based on PFKFB3 antibody-PFKFB3 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PFKFB3 antigen targets in samples.
Background: PFKFB3 is a key bifunctional enzyme responsible for both the synthesis and degradation of fructose-2,6-bisphosphate (the most potent allosteric activator of the glycolytic rate-limiting enzyme PFK-1). Consequently, its activity is one of the primary determinants of cellular glycolytic flux. Given its pivotal role in glycolysis, PFKFB3 plays a significant role in diseases such as obesity.
Synonyms: IPFK2; PFK2; iPFK-2, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Applications: Mouse 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) ELISA kit-Quantitative competitive is used to quantify PFKFB3 in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of mouse, providing data to support research in a wide range of areas, including inflammation, metabolism, obesity, and others.
Research Area: Inflammation; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate
Sensitivity: 0.1 ng/mL
Cross-reactivity: No significant cross-reactivity or interference was observed.
Recovery: 94-103%
Storage: Store at 2-8°C.

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