Mouse pro-opiomelanocortin (POMC) ELISA kit (5.0-100 ng/mL)

Cat. No.: 0126WXX-1442

Assay Type: Quantitative competitive ELISA

Target Species: Mouse

Assay Target: POMC

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Mouse pro-opiomelanocortin (POMC) ELISA kit (5.0-100 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Mouse pro-opiomelanocortin (POMC) ELISA kit (5.0-100 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of POMC in mouse samples with a range of 5.0-100 ng/mL and a minimum detectable dose (sensitivity) of 1.0 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This POMC ELISA kit utilizes a competitive enzyme immunoassay technique involving a polyclonal anti-POMC antibody and a POMC-HRP conjugate. Samples and buffers are incubated with the conjugate in a pre-coated plate for one hour, where sample POMC competes with the HRP-conjugate for limited antibody binding sites. After five wash cycles, an HRP substrate is introduced to form a blue product, turning yellow upon adding the stop solution. The resulting color intensity at 450 nm is inversely proportional to the POMC concentration.

Background: POMC, a highly complex precursor polypeptide belonging to the POMC family and encoded at chromosomal location 2p23.3, serves as a central hub in the endocrine regulation of energy homeostasis and systemic metabolism. Synthesized as a secreted protein that undergoes extensive peptide hormone processing, POMC is vital for the neuropeptide signaling pathways that govern appetite regulation, glucose homeostasis, and blood pressure. Its biological significance is underscored by its role in producing ACTH to stimulate adrenal cortisol release, as well as its essential binding activities at melanocortin receptors (MC3R and MC4R) to modulate feeding behavior. Crucially, genetic defects in POMC are directly linked to Pro-opiomelanocortin Deficiency (POMCD) and a heightened susceptibility to early-onset obesity—a condition defined by the excessive accumulation of body fat beyond physiological requirements. Beyond its primary metabolic functions, POMC influences diverse processes ranging from cellular pigmentation and neuropeptide transduction to the negative regulation of tumor necrosis factor production, highlighting its status as a pivotal molecular driver in the complex pathology of metabolic disorders.

Synonyms: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone; Proopiomelanocortin

Applications: Mouse pro-opiomelanocortin (POMC) ELISA kit (5.0-100 ng/mL) is used to quantify POMC in serum, plasma, cell culture supernatants, body fluid, tissue homogenate of mouse, providing data to support research in a wide range of areas, including endocrinology, obesity, and others.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 5.0-100 ng/mL

Sensitivity: 1.0 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 92-101%

Storage: Store at 2-8°C.

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