Human glutathione peroxidase 3 (GPX3) ELISA kit (1.56-100 ng/mL)

Cat. No.: 0126WXX-279

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: GPX3

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Human glutathione peroxidase 3 (GPX3) ELISA kit (1.56-100 ng/mL)

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Product Overview

Description: Human glutathione peroxidase 3 (GPX3) ELISA kit (1.56-100 ng/mL, <0.23 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GPX3 in human samples with a range of 1.56-100 ng/mL and a minimum detectable dose (sensitivity) of <0.23 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate is pre-coated with an antibody specific to the target antigen. During the reaction, the target antigen in the sample competes with a fixed amount of biotin-labeled antigen for sites on the pre-coated antibody. Unbound substances are washed away, and avidin-HRP is added. After incubation and the addition of TMB substrate, the enzymatic reaction is terminated with sulfuric acid. The color change is measured at 450 nm ± 2 nm, and the concentration is determined via the O.D. curve; the signal intensity is inversely proportional to the antigen concentration.

Background: GPX3 is a crucial antioxidant enzyme that protects cells from oxidative damage by catalyzing the reduction of hydrogen peroxide, lipid peroxides, and organic hydrogen peroxides. This enzyme belongs to the glutathione peroxidase family, primarily distributed in extracellular spaces, and plays a role in multiple biological processes, including responses to reactive oxygen species and lipid hydroperoxides. The relationship between GPX3 and obesity is a key area of exploration. Research indicates that GPX3 activity or expression levels are closely associated with oxidative stress, inflammatory responses, and pathological changes in adipose tissue. Under obese conditions, GPX3 activity is frequently suppressed, potentially diminishing the body's antioxidant capacity, exacerbating oxidative stress, and thereby promoting inflammation and insulin resistance. GPX3 may serve as a potential biomarker for obesity and its associated metabolic disorders.

Synonyms: GSHPX3; GPx-P; GSHPx-P; GPXP; Plasma glutathione peroxidase; Extracellular glutathione peroxidase; Glutathione peroxidase 3, plasma

EC NO.: 1.11.1.9

Applications: Human glutathione peroxidase 3 (GPX3) ELISA kit (1.56-100 ng/mL, <0.23 ng/mL) is used to quantify GPX3 in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids of human, providing data to support research in a wide range of areas, including enzyme & kinase, obesity, and others.

Research Area: Enzyme & kinase; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell culture supernates; Other biological fluids

Detection Range: 1.56-100 ng/mL

Sensitivity: <0.23 ng/mL

Precision (Intra-assay): CV≤5.3%

Precision (Inter-assay): CV≤7.8%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C.

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