Mouse beta-3 adrenergic receptor (ADRB3) ELISA kit (15.6-1000 pg/mL)

Cat. No.: OB0525WXX-340

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: ADRB3

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Mouse beta-3 adrenergic receptor (ADRB3) ELISA kit (15.6-1000 pg/mL)

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Product Overview

Description: Mouse beta-3 adrenergic receptor (ADRB3) ELISA kit (15.6-1000 pg/mL) is a ready-to-use ELISA kit for analyzing mouse ADRB3 levels with a detection range of 15.6-1000 pg/mL and a sensitivity of <5.9 pg/mL.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific for ADRB3. Standards or samples are then added to the appropriate microtiter plate wells along with a biotin-conjugated antibody preparation specific for ADRB3. Avidin conjugated to HRP is then added to each microplate well and incubated. Only wells containing ADRB3, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change after addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ADRB3 in the sample is then determined by comparing the OD of the sample to a standard curve.

Background: ADRB3, the β3-adrenergic receptor, regulates lipolysis, thermogenesis in brown adipose tissue, and vasodilation. β3-adrenergic receptor agonists have been found to have significant anti-obesity and anti-diabetic effects. ADRB3 is an attractive target in anti-obesity research.

Synonyms: β3AR; β3-AR; Beta-3 adrenoceptor

Applications: Mouse beta-3 adrenergic receptor (ADRB3) ELISA kit (15.6-1000 pg/mL) is designed for the in vitro quantitative analysis of ADRB3 levels in mouse tissue homogenates, and other biological fluid samples.

Research Area: Thermogenesis; Lipolysis; Signaling pathways; Metabolism; Adipocyte differentiation; Signal transduction; Obesity

Specification

Sample Type: Mouse Tissue homogenates; Other biological fluids

Detection Range: 15.6-1000 pg/mL

Sensitivity: <5.9 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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