Rat butyrylcholinesterase (BCHE) ELISA kit (2.47-200 ng/mL)

Cat. No.: OB0625WXX-235

Assay Type: Quantitative competitive ELISA

Target Species: Rat

Assay Target: BCHE

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Rat butyrylcholinesterase (BCHE) ELISA kit (2.47-200 ng/mL)

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Product Overview

Description: Rat butyrylcholinesterase (BCHE) ELISA kit (2.47-200 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of BCHE in rat with a range of 2.47-200 ng/mL and a specificity of <0.91 ng/mL.

Assay Principle: Monoclonal antibodies specific for BCHE have been pre-coated on microtiter plates. Biotin-labeled BCHE and unlabeled BCHE (standards or samples) undergo a competitive inhibitory reaction with the pre-coated BCHE-specific antibody. After incubation, unbound conjugates are washed away. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter well and incubated. The amount of HRP conjugate bound is inversely proportional to the concentration of BCHE in the sample. Upon addition of the substrate solution, the intensity of color development is inversely proportional to the concentration of BCHE in the sample.

Background: BCHE is a non-specific cholinesterase that has broad substrate specificity and is involved in detoxification reactions, drug metabolism, among other processes.

Synonyms: BuChE; CHE1; E1; Pseudocholinesterase; Cholinesterase; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; EC 3.1.1.8

EC NO.: 3.1.1.8

Applications: Rat butyrylcholinesterase (BCHE) ELISA kit (2.47-200 ng/mL) is used to quantify BCHE in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples of rat, providing data to support a wide range of studies.

Research Area: Metabolic pathway

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 2.47-200 ng/mL

Sensitivity: <0.91 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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