Human transient receptor potential cation channel subfamily V; member 1 (TRPV1) ELISA kit (0.312-20 ng/mL)

Cat. No.: 0126WXX-2007

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: TRPV1

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Human transient receptor potential cation channel subfamily V; member 1 (TRPV1) ELISA kit (0.312-20 ng/mL)

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Product Overview

Description: Human transient receptor potential cation channel subfamily V; member 1 (TRPV1) ELISA kit (0.312-20 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of TRPV1 in human samples with a range of 0.312-20 ng/mL and a minimum detectable dose (sensitivity) of <0.121 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit utilizes the sandwich enzyme immunoassay technique. The microtiter plate is pre-coated with an antibody specific to TRPV1. Standards or samples are introduced into the appropriate wells along with a biotin-conjugated detection antibody specific for TRPV1. Next, avidin-conjugated HRP is added to each microplate well and incubated. After the TMB substrate solution is added, a colorimetric change will be observed only in those wells containing the complete TRPV1 sandwich complex. The reaction is terminated by the addition of a sulphuric acid solution, and the color shift is measured at 450 nm ± 10 nm. The concentration of TRPV1 in the specimens is then determined by mapping the O.D. against the standard curve.

Background: Transient receptor potential cation channel subfamily V member 1 (TRPV1) is a non-selective cation channel and receptor for capsaicin, functioning as a transducer of thermal and chemical stimuli in sensory neurons. By mediating calcium influx and downstream signaling, TRPV1 influences energy metabolism, thermogenesis, and appetite regulation. Altered TRPV1 activity has been associated with changes in metabolic rate and energy balance, linking it to obesity and related metabolic disorders.

Synonyms: Vanilloid receptor subtype 1; Capsaicin receptor; Osm-9-like TRP channel 1; Vanilloid receptor 1; VR1; OTRPC1

Applications: Human transient receptor potential cation channel subfamily V, member 1 (TRPV1) ELISA kit (0.312-20 ng/mL) is used to quantify TRPV1 in tissue homogenates, cell lysates, and other biological fluids of humans, providing data to support research in a wide range of areas, including signal transduction, obesity, and others.

Research Area: Signal transduction; Obesity

Specification

Sample Type: Tissue homogenates; Cell lysates; Other biological fluids

Detection Range: 0.312-20 ng/mL

Sensitivity: <0.121 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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