Rat adiponectin (ADP) ELISA kit (0.156-10 ng/mL, <10 pg/mL)

Cat. No.: OB0525WXX-226

Assay Type: Quantitative ELISA

Target Species: Rat

Assay Target: ADP

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Rat adiponectin (ADP) ELISA kit (0.156-10 ng/mL, <10 pg/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Rat adiponectin (ADP) ELISA kit (0.156-10 ng/mL, <10 pg/mL) is a ready-to-use ELISA kit for analyzing rat ADP levels with a detection range of 0.156-10 ng/mL. Its sensitivity is less than 10 pg/mL.

Assay Principle: To detect rat ADP, add the standard and sample to the wells, followed by the biotinylated detection antibody. The wells are washed with PBS or TBS buffer, and ABC-HRP is added. Unbound ABC-HRP is washed with PBS or TBS buffer, and TMB is added. tMB is an HRP substrate that catalyzes the production of a blue product, which turns yellow upon addition of an acidic termination solution. The absorbance of the yellow product at 450 nm is linearly proportional to the rat ADP in the sample. The absorbance of the yellow product in each well is read using a plate reader, and the readings from the sample wells are compared to a standard curve to determine the concentration of rat ADP in the sample.

Background: ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.

Synonyms: ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin

Applications: Rat adiponectin (ADP) ELISA kit (0.156-10 ng/mL, <10 pg/mL) is designed for the in vitro quantitative analysis of ADP levels in rat cell culture supernatants, serum, plasma (heparin, edta), and urine samples.

Research Area: Hormone research; Lipid metabolism; Cell differentiation; Obesity

Specification

Sample Type: Cell culture supernatants; Serum; Plasma (Heparin; edta); Urine

Detection Range: 0.156-10 ng/mL

Sensitivity: <10 pg/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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