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Mouse agouti-related protein (AGRP) ELISA kit (7-1500 ng/L)

Cat. No.: OB0525WXX-393
Assay Type: Quantitative sandwich ELISA
Target Species: Mouse
Assay Target: AGRP
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Mouse agouti-related protein (AGRP) ELISA kit (7-1500 ng/L)
INQUIRY Datasheet MSDS
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Product Overview

Description: Mouse agouti-related protein (AGRP) ELISA kit (7-1500 ng/L) is a ready-to-use ELISA kit for analyzing mouse AGRP levels with a detection range of 7-1500 ng/L and a sensitivity of 3.53 ng/L.
Assay Principle: The plate is pre-coated with mouse AGRP antibody. AGRP added to the sample binds to the antibody coated on the wells. Biotinylated mouse AGRP antibody is then added, which binds to AGRP in the sample. Streptavidin-HRP is then added to bind to the biotinylated AGRP antibody. After incubation, unbound streptavidin-HRP is washed out. The substrate solution is then added, and the color is developed in proportion to the amount of mouse AGRP. The reaction is terminated by the addition of an acidic termination solution, and the absorbance is measured at 450 nm.
Background: AGRP plays a role in body weight homeostasis, is involved in the control of eating behavior through the central melanocortin system, and acts as an inverse agonist of MC3R and MC4R, thereby inhibiting their constitutive activity. Genetic variants in AGRP may be responsible for obesity.
Synonyms: Agouti-related peptide; ASIP2
Applications: Mouse agouti-related protein (AGRP) ELISA kit (7-1500 ng/L) is designed for the in vitro quantitative analysis of AGRP levels in mouse serum, plasma, cell culture supernates, cell lysates, and tissue homogenate samples.
Research Area: Hormone research; Eating behavior; Signaling pathway; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates
Detection Range: 7-1500 ng/L
Sensitivity: 3.53 ng/L
Cross-reactivity: No significant cross-reactivity or interference was observed.
Storage: Store at 2-8°C.

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