Human agouti-related protein (AGRP) ELISA kit (31.25-2000 pg/mL, 0.002 pg/mL)

Cat. No.: OB0525WXX-381

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: AGRP

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Human agouti-related protein (AGRP) ELISA kit (31.25-2000 pg/mL, 0.002 pg/mL)

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Product Overview

Description: Human agouti-related protein (AGRP) ELISA kit (31.25-2000 pg/mL, 0.002 pg/mL) is a ready-to-use ELISA kit for analyzing human AGRP levels with a detection range of 31.25-2000 pg/mL and a sensitivity of 0.002 pg/mL.

Assay Principle: The assay utilizes a quantitative sandwich enzyme immunoassay technique in which an antibody specific for AGRP is pre-coated on a microtiter plate. Standards and samples are pipetted into the wells, and any AGRP is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific to AGRP is added to the wells. After washing, avidin-conjugated HRP is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of AGRP bound in the first step. The color development is stopped, and the intensity of the color is measured.

Background: AGRP plays a role in body weight homeostasis, is involved in the control of eating behavior through the central melanocortin system, and acts as an inverse agonist of MC3R and MC4R, thereby inhibiting their constitutive activity. Genetic variants in AGRP may be responsible for obesity.

Synonyms: Agouti-related peptide; ASIP2

Applications: Human agouti-related protein (AGRP) ELISA kit (31.25-2000 pg/mL, 0.002 pg/mL) is designed for the in vitro quantitative analysis of AGRP levels in human serum, plasma, cell culture supernates, and urine. samples.

Research Area: Hormone research; Eating behavior; Signaling pathway; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernates; Urine.

Detection Range: 31.25-2000 pg/mL

Sensitivity: 0.002 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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