Rat apolipoprotein C-III (apo-CIII) ELISA kit (6.25-400 ng/mL)

Cat. No.: OB0625WXX-165

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: apo-CIII

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Rat apolipoprotein C-III (apo-CIII) ELISA kit (6.25-400 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Rat apolipoprotein C-III (apo-CIII) ELISA kit (6.25-400 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of apo-CIII in rats with a range of 6.25-400 ng/mL and a specificity of 3.75 ng/mL.

Assay Principle: The micro ELISA plates in this kit are pre-coated with an antibody specific for Apo-CIII. Standards or samples are added to the corresponding wells of the microtiter plate and bound to the specific antibody. Biotinylated detection antibody specific for Apo-CIII and Avidin-Horseradish Peroxidase (HRP) conjugate are then added sequentially to each microplate well and incubated. The free components are washed away. Substrate solution is added to each well. Only wells containing Apo-CIII, biotinylated detection antibody, and avidin-HRP conjugate will appear blue. Upon addition of sulfuric acid solution, the enzyme-substrate reaction terminates, and the color changes to yellow. Optical density (OD) is measured spectrophotometrically at 450 nm ± 2 nm. The OD value is proportional to the concentration of Apo-CIII. The concentration of Apo-CIII in the sample can be calculated by comparing the OD value of the sample with the standard curve.

Background: Apolipoprotein C-III is secreted by the liver and small intestine. It is a very low-density lipoprotein (VLDL) protein that inhibits lipoprotein lipase and hepatic lipase. Studies have found that it plays a key role in the metabolism of triglyceride-rich lipoproteins (TRLs).

Synonyms: ApoC-III ; ApoCIII; Apo C-III; Apo C III; Apolipoprotein CIII; APOC3

Applications: Rat apolipoprotein C-III (apo-CIII) ELISA kit (6.25-400 ng/mL) is used to quantify apo-CIII in serum, plasma, biological fluid samples of rat, providing data to support a wide range of studies.

Research Area: Lipid metabolism; Fatty acid biosynthesis; Obesity research

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 6.25-400 ng/mL

Sensitivity: 3.75 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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