Mouse adrenocorticotropic hormone (ACTH) ELISA kit (0.312-20 ng/mL)

Cat. No.: OB0525WXX-180

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: ACTH

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Mouse adrenocorticotropic hormone (ACTH) ELISA kit (0.312-20 ng/mL)

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Product Overview

Description: Mouse adrenocorticotropic hormone (ACTH) ELISA kit (0.312-20 ng/mL) is a ready-to-use ELISA kit for analyzing mouse ACTH levels with a detection range of 0.312-20 ng/mL and a sensitivity of 0.126 ng/mL.

Assay Principle: The microtiter plates provided in this kit are pre-coated with specific ACE antibody, and standards or samples are then added to the appropriate microtiter plate wells along with the biotin-conjugated specific ACE antibody preparation. Avidin conjugated to HRP is then added to each microplate well and incubated. Only wells containing ACE, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change upon addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ACE in the sample is then determined by comparing the OD of the sample with the standard curve.

Background: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase in body weight beyond the limitations of skeletal and physical requirements, as a result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency, and red hair.

Synonyms: ACTH; Corticotropin; Adrenocorticotropin; Adrenocorticotropic hormone; Pomc1; Pomc-1; Beta-LPH; AlphaMSH; beta-MSH; Gamma-LPH; Alpha-MSH; Gamma-MSH

Applications: Mouse adrenocorticotropic hormone (ACTH) ELISA kit (0.312-20 ng/mL) is designed for the in vitro quantitative analysis of ACTH levels in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples.

Research Area: Hormone research; Metabolites; Signaling pathway; Glucose homeostasis; Regulation of appetite; Obesity

Specification

Sample Type: Mouse serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 0.312-20 ng/mL

Sensitivity: 0.126 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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