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Vitamin B12 test kit

Rat insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (78.13-5000 pg/mL)

Cat. No.: 0126WXX-494
Assay Type: Quantitative ELISA
Target Species: Rat
Assay Target: IGFBP-2
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Rat insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (78.13-5000 pg/mL)
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Product Overview

Description: Rat insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (78.13-5000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of IGFBP-2 in rat samples with a range of 78.13-5000 pg/mL and a minimum detectable dose (sensitivity) of 20 pg/mL. The kit is highly sensitive and easy to use.
Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific to IGFBP-2. Standards or samples are added to the wells along with a biotin-conjugated detection antibody and avidin-HRP, followed by incubation. TMB substrate is then added to each well. Only wells containing the IGFBP-2 complex will exhibit a color change. The reaction is terminated by a sulphuric acid solution, and the absorbance is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of IGFBP-2 is determined by comparing the O.D. of the samples to the standard curve.
Background: IGFBP-2 is a multifunctional molecule that plays a crucial role in human growth, metabolism, and cellular processes by regulating the biological activity of insulin-like growth factors (IGFs). Primarily through binding to IGF proteins, IGFBP-2 prolongs their half-life in the body and modulates their interactions with cell surface receptors, thereby inhibiting or enhancing the pro-growth effects of IGFs. The role of IGFBP-2 in obesity has garnered increasing attention. Its reduced levels are closely associated with obesity, dyslipidemia, and insulin resistance.
Synonyms: IBP-2; IGF-binding protein 2; IGFBP2; BP2, IBP2; Insulin-Like Growth Factor Binding Protein 2, 36kDa; Insulin-Like Growth Factor-Binding Protein 2
Applications: Rat insulin like growth factor binding protein 2 (IGFBP-2) ELISA kit (78.13-5000 pg/mL) is used to quantify IGFBP-2 in rat samples, providing data to support research in a wide range of areas, including immunity, endocrinology, signal transduction, obesity, and others.
Research Area: Immunity; Endocrinology; Signal transduction; Obesity

Specification

Sample Type: Rat samples
Detection Range: 78.13-5000 pg/mL
Sensitivity: 20 pg/mL
Precision (Intra-assay): CV<8%
Precision (Inter-assay): CV<10%
Cross-reactivity: No significant cross-reactivity or interference was observed.
Storage: Store at 2-8°C.
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