Pigeon hormone sensitive lipase (LIPE) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-425

Assay Type: Quantitative sandwich ELISA

Target Species: Pigeon

Assay Target: LIPE

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Pigeon hormone sensitive lipase (LIPE) ELISA kit-Quantitative sandwich

INQUIRY Datasheet MSDS

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Product Overview

Description: Pigeon hormone sensitive lipase (LIPE) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LIPE in pigeon samples. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a two-site sandwich ELISA to quantify LIPE. A specific capture antibody is pre-coated onto a microplate. Standards and samples are pipetted into the wells, where LIPE is bound by the immobilized antibody. After washing, a biotin-conjugated detection antibody is added. Following another wash, Streptavidin-HRP is introduced. After a final wash to remove any unbound avidin-enzyme reagent, a substrate solution is added; color develops in proportion to the amount of LIPE bound. The reaction is stopped, and the final color intensity is measured.

Background: LIPE, as a rate-limiting lipolytic enzyme, plays a pivotal role in lipid metabolism. It primarily hydrolyzes triglycerides stored in adipose tissue and cardiac muscle, converting them into free fatty acids. LIPE activity undergoes precise hormonal regulation: it is rapidly activated via cAMP-dependent phosphorylation under catecholamine influence, while insulin promotes its dephosphorylation and inactivation. Additionally, LIPE functions in steroid-producing tissues, converting cholesterol esters into cholesterol for steroid hormone synthesis. LIPE plays a pivotal role in the onset and progression of obesity, serving as the key rate-limiting enzyme in lipolysis. Its core function is to promote fat breakdown, releasing triglycerides stored within adipocytes for the body to utilize as energy. Obesity is frequently associated with abnormal regulation of LIPE activity within fat cells. Imbalances in its activity and regulation constitute a major molecular mechanism underlying fat accumulation and obesity.

Synonyms: HSL; LHS; Lipase, hormone sensitive; EC 3.1.1.79

EC NO.: 3.1.1.79

Applications: Pigeon hormone sensitive lipase (LIPE) ELISA kit is used to quantify LIPE in serum, plasma, and other biological fluids of pigeons, providing data to support research in a wide range of areas, including metabolism, obesity, and others.

Research Area: Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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