Human pro-opiomelanocortin (POMC) ELISA kit (0.16-10 ng/mL)

Cat. No.: 0126WXX-1429

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: POMC

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Human pro-opiomelanocortin (POMC) ELISA kit (0.16-10 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Human pro-opiomelanocortin (POMC) ELISA kit (0.16-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of POMC in human samples with a range of 0.16-10 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This ELISA kit utilizes the sandwich-ELISA principle. The microplate provided is pre-coated with an antibody specific to human POMC. Standards or samples are added to the wells to bind with the specific antibody. Subsequently, a biotinylated detection antibody specific for human POMC and an avidin-HRP conjugate are added successively to each well and incubated. After washing away free components, a substrate solution is added. Only wells containing the human POMC complex will turn blue, eventually shifting to yellow upon the addition of a stop solution. The optical density (O.D.) is measured spectrophotometrically at 450 nm ± 2 nm, where the O.D. value is directly proportional to the concentration of human POMC.

Background: POMC, a highly complex precursor polypeptide belonging to the POMC family and encoded at chromosomal location 2p23.3, serves as a central hub in the endocrine regulation of energy homeostasis and systemic metabolism. Synthesized as a secreted protein that undergoes extensive peptide hormone processing, POMC is vital for the neuropeptide signaling pathways that govern appetite regulation, glucose homeostasis, and blood pressure. Its biological significance is underscored by its role in producing ACTH to stimulate adrenal cortisol release, as well as its essential binding activities at melanocortin receptors (MC3R and MC4R) to modulate feeding behavior. Crucially, genetic defects in POMC are directly linked to Pro-opiomelanocortin Deficiency (POMCD) and a heightened susceptibility to early-onset obesity—a condition defined by the excessive accumulation of body fat beyond physiological requirements. Beyond its primary metabolic functions, POMC influences diverse processes ranging from cellular pigmentation and neuropeptide transduction to the negative regulation of tumor necrosis factor production, highlighting its status as a pivotal molecular driver in the complex pathology of metabolic disorders.

Synonyms: POC; NPP; ProOMC; ACTH; Pro-OMC; Pro opiomelanocortin; Corticotropin-lipotropin; Corticotropin; Adrenocorticotropic hormone; Proopiomelanocortin

Applications: Human pro-opiomelanocortin (POMC) ELISA kit (0.16-10 ng/mL) is used to quantify POMC in serum, plasma, and other biological fluids of human, providing data to support research in a wide range of areas, including endocrinology, obesity, and others.

Research Area: Endocrinology; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 0.16-10 ng/mL

Sensitivity: 0.1 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 86-111%

Storage: Store at 2-8°C.

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