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Mouse adiponectin (ADP) ELISA kit (0.156-10 ng/mL, <0.063 ng/mL)
Cat. No.:
OB0525WXX-264
Assay Type:
Quantitative sandwich ELISA
Target Species:
Mouse
Assay Target:
ADP
Size:
Add to basket
Product Overview
Description:
Mouse adiponectin (ADP) ELISA kit (0.156-10 ng/mL, <0.063 ng/mL) is a ready-to-use ELISA kit for analyzing mouse ADP levels with a detection range of 0.156-10 ng/mL. Its sensitivity is less than 0.063 ng/mL.
Assay Principle:
The microtiter plates provided in this kit are pre-coated with ADP-specific antibodies. Standards or samples are then added to the appropriate wells, followed by the addition of biotin-conjugated ADP-specific antibody. HRP-conjugated avidin is then added to each well and incubated. Only the wells containing ADP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change upon addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is then measured spectrophotometrically at 450 nm ± 10 nm. The concentration of ADP in the sample can then be determined by comparing the OD value of the sample to a standard curve.
Background:
ADP is an important adipokine involved in the regulation of fat metabolism and insulin sensitivity, exhibiting antidiabetic, anti-atherosclerotic, and anti-inflammatory properties. It increases glucose utilization and fatty acid burning, stimulates AMPK phosphorylation and activation in liver and skeletal muscle, and may play a role in cell growth, angiogenesis, and tissue remodeling.
Synonyms:
ADIPOQ; ACDC; APM1; Adipocyte C1q and collagen domain-containing protein; ACRP30; Adipose most abundant gene transcript 1 protein; apM-1; Gelatin-binding protein; Adiponectin
Applications:
Mouse adiponectin (ADP) ELISA kit (0.156-10 ng/mL, <0.063 ng/mL) is designed for the in vitro quantitative analysis of ADP levels in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples.
Research Area:
Hormone research; Lipid metabolism; Cell differentiation; Obesity
Specification
Sample Type:
Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids
Detection Range:
0.156-10 ng/mL
Sensitivity:
<0.063 ng/mL
Precision (Intra-assay):
CV<10%
Precision (Inter-assay):
CV<12%
Cross-reactivity:
No significant cross-reactivity or interference was observed.
Storage:
Store at 2-8°C.
