Rat apolipoprotein C-III (apo-CIII) ELISA kit (1.56-100 ng/mL, <0.65 ng/mL)

Cat. No.: OB0625WXX-166

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: apo-CIII

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Rat apolipoprotein C-III (apo-CIII) ELISA kit (1.56-100 ng/mL, <0.65 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Rat apolipoprotein C-III (apo-CIII) ELISA kit (1.56-100 ng/mL, <0.65 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of apo-CIII in rats with a range of 1.56-100 ng/mL and a specificity of <0.65 ng/mL.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific for Apo-CIII. Standards or samples are added to the corresponding microtiter plate wells and the biotin-conjugated Apo-CIII-specific antibody. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter well and incubated. Only those wells containing Apo-CIII, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change upon addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The OD of the sample is then compared to a standard curve to determine the concentration of Apo-CIII in the sample.

Background: Apolipoprotein C-III is secreted by the liver and small intestine. It is a very low-density lipoprotein (VLDL) protein that inhibits lipoprotein lipase and hepatic lipase. Studies have found that it plays a key role in the metabolism of triglyceride-rich lipoproteins (TRLs).

Synonyms: ApoC-III ; ApoCIII; Apo C-III; Apo C III; Apolipoprotein CIII; APOC3

Applications: Rat apolipoprotein C-III (apo-CIII) ELISA kit (1.56-100 ng/mL, <0.65 ng/mL) is used to quantify apo-CIII in serum, plasma, and other biological fluid samples of rats, providing data to support a wide range of studies.

Research Area: Lipid metabolism; Fatty acid biosynthesis; Obesity research

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 1.56-100 ng/mL

Sensitivity: <0.65 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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