Monkey leptin (LEP) ELISA kit (2.5-40 ng/mL)

Cat. No.: 0126WXX-816

Assay Type: Quantitative competitive ELISA

Target Species: Monkey

Assay Target: LEP

Size:

Monkey leptin (LEP) ELISA kit (2.5-40 ng/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Monkey leptin (LEP) ELISA kit (2.5-40 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LEP in monkey samples with a range of 2.5-40 ng/mL and a minimum detectable dose (sensitivity) of 2.5 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit employs a competitive inhibition enzyme Immunoassay technique. The microtiter plate is pre-coated with an antibody specific to LEP. A competitive inhibition reaction occurs between the LEP in standards or samples and a fixed amount of biotin-conjugated LEP for the pre-coated antibody sites. Following a wash step, Streptavidin-conjugated HRP is added to the wells. Upon the addition of the substrate solution, color development is inversely proportional to the amount of LEP present in the sample, which is then quantified by measuring the color intensity.

Background: LEP is a hormone that plays a crucial role in regulating body fat. Secreted by fat cells, it functions as part of a signaling pathway that controls the extent of fat storage in the human body. This protein belongs to the leptin family and possesses dual functions as both a growth factor and a hormone. LEP's primary functions include: regulating food intake, controlling energy expenditure, participating in glucose homeostasis regulation, modulating adipocyte differentiation, and influencing fatty acid metabolism. Obesity research frequently focuses on deciphering the mechanisms of leptin resistance and developing countermeasures, including the development of drugs that enhance brain sensitivity to leptin or the exploration of alternative pathways for regulating appetite and energy balance. Understanding leptin-mediated signaling pathways is central to this research.

Synonyms: OB; OBS; Obesity Homolog; Obesity Factor; Obese Protein

Applications: Monkey leptin (LEP) ELISA kit (2.5-40 ng/mL) is used to quantify LEP in serum, plasma, and other biological fluids of monkey, providing data to support research in a wide range of areas, including metabolic pathway, endocrinology, cardiovascular biology, obesity, etc.

Research Area: Metabolic pathway; Endocrinology; Cardiovascular biology; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Detection Range: 2.5-40 ng/mL

Sensitivity: 2.5 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out