LipoKnoxa™ Human OTOP1 shRNA Lentiviral Particle (Silencing)

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX610-1	GGGCATGCAAAGGATATTATC	CDS	Inquiry
V0126XX610-2	TGGTGGTGGTATACCTGATTC	CDS	Inquiry
V0126XX610-3	CCCTCTTTGAGGTCTATTGTA	CDS	Inquiry
V0126XX610-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human OTOP1 shRNA Lentiviral Particle (Silencing) targets otopetrin 1, a proton channel involved in modulating brown adipose tissue thermogenesis. Utilizing an advanced RNAi framework with a choice of U6 or miR30-based systems, researchers can evaluate the role of proton transport in energy expenditure. Every batch undergoes comprehensive quality control screening for functional titer, sequence identity, and mycoplasma testing, ensuring scientific robustness and experimental safety.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: OTOP1

Full Name: Otopetrin 1

Gene ID: 133060

RefSeq ID-1: NP_819056.1

RefSeq ID-2: NM_177998.3

Summary: Originally discovered for its role in maintaining balance through the formation of inner ear minerals, OTOP1 has a surprising secondary function in adipose tissue. It acts as a proton channel that attenuates the inflammation induced by obesity. In fat cells, OTOP1 is part of a counter-inflammatory pathway that helps the body adapt to the metabolic stress of excess weight.

LipoKnoxa™ Human OTOP1 shRNA Lentiviral Particle (Silencing)

Product Overview

Specification

Target Profile

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