LipoKnoxa™ Human IL1B shRNA Lentiviral Particle (Silencing)

Sub Cat. No.	TargetSeq	Region	Inquiry
V0126XX511-1	AGCAACCGCTTCCCTATTTAT	3' UTR	Inquiry
V0126XX511-2	TCACCTCTCCTACTCACTTAA	3' UTR	Inquiry
V0126XX511-3	ATCAATAACAAGCTGGAATTT	CDS	Inquiry
V0126XX511-4	Other		Inquiry

Product Overview

Description: LipoKnoxa™ Human IL1B shRNA Lentiviral Particle (Silencing) is engineered for the stable knockdown of interleukin-1 beta, a driver of chronic inflammation in obesity. Utilizing an advanced RNAi framework with optimized U6 or miR30-based shRNA delivery, this tool assists in uncovering inflammatory pathways. Every batch is subjected to rigorous quality control, including functional titer, sterility, and mycoplasma testing.

Production Cell Line: HEK293T

Promoter: U6; CMV; EF1α; CAG; UBC

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Comprehensive sequencing and PCR analyses were performed to verify the accurate genomic sequence of all viral preparations.

Sterility Test: Confirmed sterile via rigorous microbial analysis; free of bacterial and fungal contamination.

Mycoplasma Test: Rigorous quality control testing has confirmed the absence of mycoplasma contamination in this viral preparation.

Other QC: Customized supplementary testing and in vitro/in vivo assessments are available to verify post-silencing gene expression and biological functionality, ensuring the viral preparations meet the specific potency requirements of your gene interference project.

Storage: Store at -80°C for long-term preservation. Immediate transfer upon delivery is required to prevent loss of viral activity.

Stability: Shelf life: 6–12 months at -80°C (extended stability up to 2 years). Post-thaw: Stable at 4°C for 2–3 weeks without significant degradation of functional titers.

Shipping Condition: Products are delivered on dry ice to maintain the cold chain. Upon arrival, please ensure the presence of dry ice and transfer the vials immediately to -80°C storage.

Handling Notes: Avoid repeated freeze-thaw cycles. Aliquot into low-protein-binding tubes immediately after receipt to maintain optimal activity. For safety and contamination control, all viral handling must be performed inside a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: We ensure product integrity through rigorous QC, but we do not guarantee results in specific research contexts. Proper storage, handling, and total compliance with biosafety laws and safety protocols remain the sole responsibility of the user.

Target Profile

Gene Name: IL1B

Full Name: Interleukin 1 beta

Gene Symbol: IL-1; IL1F2; IL1beta; IL1-BETA

Gene ID: 3553

RefSeq ID-1: NP_000567.1

RefSeq ID-2: NM_000576.3

Summary: The IL1B gene produces a foundational pro-inflammatory cytokine. Synthesized as an inactive proprotein by macrophages, it undergoes maturation via proteolytic cleavage by caspase 1. IL1B acts as a key orchestrator of the inflammatory response, influencing cell survival and tissue differentiation. In metabolic studies, the chronic induction of IL1B is a primary driver of the low-grade systemic inflammation associated with obesity and comorbid conditions like osteoarthritis and severe respiratory distress in viral infections.

LipoKnoxa™ Human IL1B shRNA Lentiviral Particle (Silencing)

Product Overview

Specification

Target Profile

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