Human peptide YY (PYY) ELISA kit-Quantitative sandwich (3-900 pg/mL)

Cat. No.: 0126WXX-1654

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: PYY

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Human peptide YY (PYY) ELISA kit-Quantitative sandwich (3-900 pg/mL)

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Product Overview

Description: Human peptide YY (PYY) ELISA kit-Quantitative sandwich (3-900 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PYY in human samples with a range of 3-900 pg/mL and a minimum detectable dose (sensitivity) of 1.56 pg/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microplate is pre-coated with a human PYY capture antibody. PYY in the sample binds to the immobilized antibodies, followed by the addition of a biotinylated human PYY detection antibody and streptavidin-HRP. After incubation and a washing step to remove unbound reagents, a substrate solution is added. Color develops in direct proportion to the concentration of human PYY. The reaction is terminated with an acidic stop solution, and the absorbance is measured at 450 nm.

Background: PYY (Peptide YY), a critical gut peptide and member of the NPY family located at chromosomal position 17q21.1, serves as a fundamental regulator in the physiological landscape of obesity and digestive homeostasis. Secreted into the extracellular region as two distinct isoforms through alternative splicing, this neuropeptide hormone exerts its biological influence primarily through G-protein coupled receptor signaling pathways to modulate eating behavior and appetite regulation. Its multifaceted role in the gastrointestinal tract includes the inhibition of exocrine pancreatic secretion, the mediation of vasoconstrictory actions, and the suppression of jejunal and colonic motility, thereby slowing digestion to enhance satiety. Beyond its classical endocrine functions in feeding behavior, PYY is also implicated in broader cellular processes such as cell proliferation and cytoskeleton organization.

Synonyms: Peptide YY-like

Applications: Human peptide YY (PYY) ELISA kit-Quantitative sandwich (3-900 pg/mL) is used to quantify PYY in serum, plasma, cell culture supernatants, ascites, tissue homogenates, and other biological fluids of humans, providing data to support research in a wide range of areas, including neuroscience, hepatology, obesity, and others.

Research Area: Neuro science; Hepatology; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; ascites; Tissue homogenates; Other biological fluids

Detection Range: 3-900 pg/mL

Sensitivity: 1.56 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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