Human lipoprotein lipase (LPL) ELISA kit (1.56-100 ng/mL)

Cat. No.: 0126WXX-950

Assay Type: Quantitative ELISA

Target Species: Human

Assay Target: LPL

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Human lipoprotein lipase (LPL) ELISA kit (1.56-100 ng/mL)

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Product Overview

Description: Human lipoprotein lipase (LPL) ELISA kit (1.56-100 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LPL in human samples with a range of 1.56-100 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific to the target antigen. Standards or samples are added to the appropriate wells along with a biotin-conjugated detection antibody and avidin-conjugated HRP. Following incubation and the addition of TMB substrate, only those wells containing the target complex will exhibit a colorimetric change. The enzyme-substrate reaction is terminated by a sulphuric acid solution, and the absorbance is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the target antigen in the samples is then determined by comparing the O.D. of the samples to the generated standard curve.

Background: LPL is an enzyme belonging to the lipoprotein lipase family. Its primary function is to break down triglycerides (a type of fat) in the blood. Specifically, LPL hydrolyzes triglycerides present in circulating lipoproteins such as chylomicrons and very low-density lipoproteins (VLDL). The free fatty acids and monoglycerides released during this process can be utilized by cells to generate energy or stored for later use. LPL's direct relevance to obesity research lies in its pivotal role in fat storage and metabolism. Given its central function in lipid metabolism, LPL has emerged as a key biological factor in obesity studies.

Synonyms: Clearing factor; Clearing factor lipase; EC 3.1.1.34

EC NO.: 3.1.1.34

Applications: Human lipoprotein lipase (LPL) ELISA kit (1.56-100 ng/mL) is used to quantify LPL in serum, plasma, tissue homogenates, cell culture supernatant, and other biological fluids of human, providing data to support research in a wide range of areas, including signal transduction, metabolism, obesity, etc.

Research Area: Signal Transduction; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell culture supernatants; Other biological fluids

Detection Range: 1.56-100 ng/mL

Precision (Intra-assay): CV<5.4%

Precision (Inter-assay): CV<8.6%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at -20°C.

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