Human α-melanocyte-stimulating hormone (α-MSH) ELISA kit

Cat. No.: OB0625WXX-105

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: α-MSH

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Human α-melanocyte-stimulating hormone (α-MSH) ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Human α-melanocyte-stimulating hormone (α-MSH) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of α-MSH in human with a range of 0.156-10 ng/mL and a specificity of 0.094 ng/mL.

Assay Principle: The microtiter plates provided in this kit are pre-coated with human α-MSH. During the reaction, human α-MSH in the samples or standards competes with a certain amount of human α-MSH on the solid phase support for sites on the biotinylated detection antibody that are specific for human α-MSH. Excess conjugate and unbound samples or standards are washed off the plate, and then avidin conjugated to horseradish peroxidase (HRP) is added to each microtiter well and incubated. TMB substrate solution is then added to each well. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and then the color change is measured spectrophotometrically at 450 nm ± 2 nm. The concentration of human α-MSH in the sample can then be determined by comparing the OD of the sample to a standard curve.

Background: α-MSH is an endogenous peptide hormone and neuropeptide of the melanocortin family. It is known as the most important hormone for stimulating melanogenesis and also plays a role in areas such as feeding behavior and energy homeostasis.

Synonyms: Intermedins; Alpha-melanotropin, Alpha-melanocortin; Alpha-intermedin; aMSH; Alpha-melanocyte stimulating hormone

Applications: Human α-melanocyte-stimulating hormone (α-MSH) ELISA kit is used to quantify α-MSH in serum, plasma, and biological fluid samples of humans, providing data to support a wide range of studies.

Research Area: Metabolism; Energy homeostasis; Obesity research

Specification

Sample Type: Serum; Plasma; Biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.094 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C.

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