Mouse neuropeptide Y receptor Y5 (NPY5R) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-1145

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: NPY5R

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Mouse neuropeptide Y receptor Y5 (NPY5R) ELISA kit-Quantitative sandwich

INQUIRY Datasheet MSDS

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Product Overview

Description: Mouse neuropeptide Y receptor Y5 (NPY5R) ELISA kit-Quantitative sandwich is an ELISA-based in vitro research tool designed specifically for the quantitative detection of NPY5R in mice samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.057 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The provided microtiter plate is pre-immobilized with an antibody specific to NPY5R. Standards or samples are added to the appropriate microtiter wells, followed by the addition of a biotin-conjugated detection antibody specific for NPY5R. Subsequently, avidin-conjugated HRP is introduced to each well and incubated. After the TMB substrate is added, color development occurs exclusively in wells containing the complete NPY5R sandwich. The reaction is halted by the addition of a sulphuric acid solution, and the absorbance is measured at 450 nm ± 10 nm. The concentration of NPY5R in the specimens is determined by mapping the O.D. values against the established standard curve.

Background: NPY5R is a G protein-coupled receptor that binds to neuropeptide Y (NPY) and peptide YY (PYY). These are key signaling molecules regulating appetite within the brain. When NPY5R is activated, it inhibits adenylate cyclase activity via its G protein, thereby triggering cellular responses. As a central control point regulating appetite and feeding behavior, it serves as a critical target in obesity research.

Synonyms: NPY-Y5 receptor

Applications: Mouse neuropeptide Y receptor Y5 (NPY5R) ELISA kit-Quantitative sandwich is used to quantify NPY5R in tissue homogenates and other biological fluids of mouse, providing data to support research in a wide range of areas, including signal transduction, obesity, etc.

Research Area: Signal transduction; Obesity

Specification

Sample Type: Tissue homogenates; Other biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.057 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (others).

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