Human growth hormone (GH) ELISA kit (0.5 ng/mL)

Cat. No.: 0126WXX-352

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: GH

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Human growth hormone (GH) ELISA kit (0.5 ng/mL)

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Product Overview

Description: Human growth hormone (GH) ELISA kit (0.5 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GH in human samples with a minimum detectable dose (sensitivity) of 0.5 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This GH ELISA applies a quantitative sandwich immunoassay technique. The microtiter plate is pre-coated with a monoclonal antibody specific to GH. After sample incubation and washing, an HRP-conjugated monoclonal antibody is added to "sandwich" the immobilized GH. Following a thorough wash, TMB substrate is added, reacting with the HRP to produce a color change. The reaction is terminated with sulphuric acid and measured at 450 nm. By assaying a set of six calibration standards simultaneously, a standard curve is generated to determine the GH concentration (ng/mL) in the samples based on their O.D. values.

Background: Growth hormone (GH) is a crucial endocrine hormone that plays a key role in growth regulation and metabolic control. It primarily promotes bodily growth by stimulating the liver and other tissues to secrete insulin-like growth factor-1 (IGF-1), while also enhancing amino acid uptake and protein synthesis. Deficiencies in GH can lead to growth hormone deficiency disorders, such as dwarfism. Research indicates that GH induces lipolysis through direct lipolytic effects in adipose tissue and influences obesity to a certain extent.

Synonyms: GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary growth hormone; Growth hormone, normal

Applications: Human growth hormone (GH) ELISA kit (0.5 ng/mL) is used to quantify GH in serum of human, providing data to support research in a wide range of areas, including developmental biology, obesity, endocrinology, neuro science, metabolism, and others.

Research Area: Developmental biology; Obesity; Endocrinology; Neuro science; Metabolism

Specification

Sample Type: Serum

Sensitivity: 0.5 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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