Guinea pig hormone sensitive lipase (LIPE) ELISA kit-Quantitative competitive

Cat. No.: 0126WXX-417

Assay Type: Quantitative competitive ELISA

Target Species: Guinea pig

Assay Target: LIPE

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Guinea pig hormone sensitive lipase (LIPE) ELISA kit-Quantitative competitive

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Product Overview

Description: Guinea pig hormone sensitive lipase (LIPE) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of LIPE in guinea pig samples with a range of 0.5-10 ng/mL and a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: The ELISA analytical biochemical technique is based on LIPE antibody-LIPE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect LIPE antigen targets in samples.

Background: LIPE, as a rate-limiting lipolytic enzyme, plays a pivotal role in lipid metabolism. It primarily hydrolyzes triglycerides stored in adipose tissue and cardiac muscle, converting them into free fatty acids. LIPE activity undergoes precise hormonal regulation: it is rapidly activated via cAMP-dependent phosphorylation under catecholamine influence, while insulin promotes its dephosphorylation and inactivation. Additionally, LIPE functions in steroid-producing tissues, converting cholesterol esters into cholesterol for steroid hormone synthesis. LIPE plays a pivotal role in the onset and progression of obesity, serving as the key rate-limiting enzyme in lipolysis. Its core function is to promote fat breakdown, releasing triglycerides stored within adipocytes for the body to utilize as energy. Obesity is frequently associated with abnormal regulation of LIPE activity within fat cells. Imbalances in its activity and regulation constitute a major molecular mechanism underlying fat accumulation and obesity.

Synonyms: HSL; LHS; Lipase, hormone sensitive; EC 3.1.1.79

EC NO.: 3.1.1.79

Applications: Guinea pig hormone sensitive lipase (LIPE) ELISA kit is used to quantify LIPE in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of guinea pig, providing data to support research in a wide range of areas, including metabolism, obesity, and others.

Research Area: Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Detection Range: 0.5-10 ng/mL

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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