Human fatty-acid amide hydrolase (FAAH) ELISA kit

Cat. No.: OB0625WXX-796

Assay Type: Quantitative sandwich ELISA

Target Species: Human

Assay Target: FAAH

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Human fatty-acid amide hydrolase (FAAH) ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Human fatty-acid amide hydrolase (FAAH) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FAAH in human with a range of 1.56-100 ng/mL and a specificity of 0.60 ng/mL.

Assay Principle: The microtiter plate provided in this kit is pre-coated with an antibody specific for FAAH. Standards or samples are then added to the appropriate microtiter plate wells along with the biotin-conjugated FAAH-specific antibody. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter well and incubated. Only wells containing FAAH, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change upon addition of TMB substrate solution. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of FAAH in the sample is then determined by comparing the OD of the sample to a standard curve.

Background: FAAH degrades bioactive fatty acid amides into corresponding acids, thereby terminating the signaling function of these molecules. It participates in neural signaling pathways involved in regulating feeding and is associated with obesity.

Synonyms: FAAH-1; Anandamide amidohydrolase 1; Oleamide hydrolase 1; EC 3.5.1.99

EC NO.: 3.5.1.99

Applications: Human fatty-acid amide hydrolase (FAAH) ELISA kit is used to quantify FAAH in tissue homogenates, cell lysates, and other biological fluid samples of human, providing data to support a wide range of studies.

Research Area: Enzyme and Kinase; Fatty acid metabolism; Obesity research

Specification

Sample Type: Tissue homogenates; Cell lysates; Other biological fluids

Detection Range: 1.56-100 ng/mL

Sensitivity: 0.60 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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