Mouse proinsulin (PI) ELISA kit (1-300 pmol/L)

Cat. No.: 0126WXX-1279

Assay Type: Quantitative sandwich ELISA

Target Species: Mouse

Assay Target: PI

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Mouse proinsulin (PI) ELISA kit (1-300 pmol/L)

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Product Overview

Description: Mouse proinsulin (PI) ELISA kit (1-300 pmol/L) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of PI in mouse samples with a range of 1-300 pmol/L and a minimum detectable dose (sensitivity) of 0.54 pmol/L. The kit is highly sensitive and easy to use.

Assay Principle: The ELISA analytical biochemical technique is based on PI antibody-PI antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PI antigen targets in samples.

Background: Proinsulin is the precursor molecule of insulin, synthesized in pancreatic β cells and subsequently processed into mature insulin and C-peptide. It plays a critical role in glucose homeostasis by serving as an essential intermediate in insulin biosynthesis and secretion. Under normal physiological conditions, proinsulin is efficiently converted to insulin; however, impaired processing or increased proinsulin secretion reflects β-cell dysfunction. Elevated circulating proinsulin levels are commonly observed in obesity and insulin resistance, making proinsulin a key indicator of metabolic stress and a contributing factor to the development of obesity-associated type 2 diabetes.

Synonyms: INS; Pro-Insulin; Preproinsulin

Applications: Mouse proinsulin (PI) ELISA kit (1-300 pmol/L) is used to quantify PI in serum, plasma, cell culture supernatant, cell lysates, and tissue homogenates of mouse, providing data to support research in a wide range of areas, including endocrinology, hormone metabolism, obesity, and others.

Research Area: Endocrinology; Hormone metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Cell lysates; Tissue homogenates

Detection Range: 1-300 pmol/L

Sensitivity: 0.54 pmol/L

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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