Human ghrelin (GHRL) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-167

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: GHRL

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Human ghrelin (GHRL) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Human ghrelin (GHRL) ELISA kit (0.156-10 ng/mL, 0.047 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GHRL in human samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.047 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit employs a sandwich enzyme immunoassay technique. A microplate is pre-coated with a capture antibody specific to GHRL. Standards or samples are added to the wells along with a biotin-conjugated detection antibody to form a "sandwich" complex with any GHRL present. After incubation, an avidin-HRP conjugate is introduced to each well. A final wash removes all unbound components before a TMB substrate solution is added. The enzymatic reaction is terminated with sulfuric acid, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The GHRL concentration is determined by comparing the sample absorbance (O.D.) to a standard curve.

Background: Ghrelin is a peptide hormone primarily secreted by the stomach, exerting its effects by binding to the growth hormone secretagogue receptor type 1 (GHSR). It plays multiple roles in the body, but its most well-known function is regulating energy balance and appetite. Research indicates that ghrelin levels and their regulation of appetite may be abnormal in obese individuals. It is considered a key target for developing anti-obesity drugs.

Synonyms: MTLRP; Preproghrelin; Growth hormone secretagogue receptor ligand; Appetite-regulating hormone; Growth hormone-releasing peptide; Motilin-related peptide

Applications: Human ghrelin (GHRL) ELISA kit (0.156-10 ng/mL, 0.047 ng/mL) is used to quantify GHRL in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluids of human, providing data to support research in a wide range of areas, including signal transduction, endocrinology, gastroenterology, metabolism, obesity, and others.

Research Area: Signal transduction; Endocrinology; Gastroenterology; Metabolism; Obesity

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.047 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 4°C (TMB substrate, wash buffer, stop solution) and -20°C (other reagents).

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