Canine growth hormone (GH) ELISA kit (0.05-30 ng/mL)

Cat. No.: 0126WXX-101

Assay Type: Quantitative sandwich ELISA

Target Species: Canine

Assay Target: GH

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Canine growth hormone (GH) ELISA kit (0.05-30 ng/mL)

INQUIRY Datasheet MSDS

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Product Overview

Description: Canine growth hormone (GH) ELISA kit (0.05-30 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GH in canine samples with a range of 0.05-30 ng/mL and a minimum detectable dose (sensitivity) of 0.024 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This ELISA utilizes a microplate pre-coated with a canine GH antibody. GH in the sample binds to the immobilized antibody, followed by the addition of a biotinylated canine GH detection antibody. Subsequently, streptavidin-HRP is introduced to bind the biotinylated antibody. After washing away unbound components, a substrate solution is added. Color develops in direct proportion to the concentration of canine GH. The reaction is terminated with an acidic stop solution and measured at 450 nm.

Background: Growth hormone (GH) is a crucial endocrine hormone that plays a key role in growth regulation and metabolic control. It primarily promotes bodily growth by stimulating the liver and other tissues to secrete insulin-like growth factor-1 (IGF-1), while also enhancing amino acid uptake and protein synthesis. Deficiencies in GH can lead to growth hormone deficiency disorders, such as dwarfism. Research indicates that GH induces lipolysis through direct lipolytic effects in adipose tissue and influences obesity to a certain extent.

Synonyms: GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary growth hormone; Growth hormone, normal

Applications: Canine growth hormone (GH) ELISA kit (0.05-30 ng/mL) is used to quantify GH in serum, plasma, cell culture supernates, cell lysates, and tissue homogenates of canine, providing data to support research in a wide range of areas, including developmental biology, obesity, endocrinology, neuro science, metabolism, and others.

Research Area: Developmental biology; Obesity; Endocrinology; Neuro science; Metabolism

Specification

Sample Type: Serum; Plasma; Cell culture supernates; Cell lysates; Tissue homogenates

Detection Range: 0.05-30 ng/mL

Sensitivity: 0.024 ng/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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