Bovine C-reactive protein (CRP) high sensitive ELISA kit

Cat. No.: OB0625WXX-426

Assay Type: Quantitative sandwich ELISA

Target Species: Bovine

Assay Target: CRP

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Bovine C-reactive protein (CRP) high sensitive ELISA kit

INQUIRY Datasheet MSDS

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Product Overview

Description: Bovine C-reactive protein (CRP) high sensitive ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of CRP in bovine with a range of 15.6-1000 pg/mL and a specificity of 6.9 pg/mL.

Assay Principle: The microtiter plates in this kit are pre-coated with an antibody specific for high-sensitivity CRP. Standards or samples are then added to the appropriate microtiter plate wells along with biotin-conjugated antibodies specific for high-sensitivity CRP. Avidin conjugated to horseradish peroxidase (HRP) is then added to each microtiter plate well and incubated. Upon addition of the TMB substrate solution, only wells containing high-sensitivity CRP, biotin-conjugated antibody, and enzyme-conjugated avidin will show a color change. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution, and the color change is measured spectrophotometrically at 450 nm ± 10 nm. The concentration of highly sensitive CRP in the sample can then be determined by comparing the OD of the sample to a standard curve.

Background: CRP is an acute-phase protein of hepatic origin, whose circulating concentrations rise in response to inflammation. It is considered a key plasma protein that can bind to Leptin, and its potential role in regulating leptin's function in obesity is crucial.

Synonyms: Pentraxin-related; pentraxin 1; C-RP; PTX1

Applications: Bovine C-reactive protein (CRP) high sensitive ELISA kit is used to quantify CRP in serum, plasma, tissue homogenates, cell lysates, cell culture supernates, and other biological fluid samples of bovine, providing data to support a wide range of studies.

Research Area: Inflammatory reaction; Obesity research

Specification

Sample Type: Serum; Plasma; Tissue homogenates; Cell lysates; Cell culture supernates; Other biological fluids

Detection Range: 15.6-1000 pg/mL

Sensitivity: 6.9 pg/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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