Canine neuroendocrine secretory protein 55 (NESP55) ELISA kit-Quantitative sandwich

Cat. No.: 0126WXX-234

Assay Type: Quantitative sandwich ELISA

Target Species: Canine

Assay Target: NESP55

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Canine neuroendocrine secretory protein 55 (NESP55) ELISA kit-Quantitative sandwich

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Product Overview

Description: Canine neuroendocrine secretory protein 55 (NESP55) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of NESP55 in canine samples. The kit is highly sensitive and easy to use.

Assay Principle: This assay employs a two-site sandwich ELISA technique to quantify NESP55. A microplate is pre-coated with an antibody specific to NESP55. Standards and samples are pipetted into the wells, where NESP55 is captured by the immobilized antibody. After washing away unbound substances, a biotin-conjugated detection antibody specific for NESP55 is added. Following another wash, streptavidin-conjugated HRP is introduced. After a final wash to remove any unbound reagents, a substrate solution is added; color develops in direct proportion to the amount of NESP55 bound in the initial step. The reaction is terminated, and the color intensity is measured.

Background: NESP55 is a protein expressed in multiple neuroendocrine tissues, particularly in the pancreas, adrenal medulla, and hypothalamus. It belongs to a protein family known as NESP, whose members are genetically closely linked and whose expression is regulated by a specific epigenetic mechanism—imprinted genes. As an imprinted gene product, its expression is influenced by parental origin and may serve as a potential regulator of energy balance, obesity, and others.

Synonyms: GNAS; AHO; GSA; GSP; POH; GPSA; NESP; GNAS1; PHP1A; PHP1B; PHP1C; C20orf45

Applications: Canine neuroendocrine secretory protein 55 (NESP55) ELISA kit is used to quantify NESP55 in serum, plasma, and other biological fluids of canine, providing data to support research in a wide range of areas, including endocrinology, epigenetic regulation, obesity, and others.

Research Area: Endocrinology; Epigenetic regulation; Obesity

Specification

Sample Type: Serum; Plasma; Other biological fluids

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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