Bovine fibroblast growth factor 21, FGF21 ELISA kit (62.5-4000 pg/mL)

Cat. No.: OB0625WXX-880

Assay Type: Quantitative sandwich ELISA

Target Species: Bovine

Assay Target: FGF21

Size:

Bovine fibroblast growth factor 21, FGF21 ELISA kit (62.5-4000 pg/mL)

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Bovine fibroblast growth factor 21, FGF21 ELISA kit (62.5-4000 pg/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of FGF21 in bovine with a range of 62.5-4000 pg/mL and a specificity of <15.6 pg/mL.

Assay Principle: An antibody specific for FGF21 has been pre-coated on the microtiter plate. Standards and samples are pipetted into the wells, and any FGF21 present is bound by the immobilized antibody. After removal of unbound material, a biotin-conjugated antibody specific for FGF21 is added to the wells. After washing, avidin-conjugated horseradish peroxidase (HRP) is added to the wells. After washing to remove unbound avidin-enzyme reagent, a substrate solution is added to the wells, and the color develops in proportion to the amount of FGF21 bound in the first step. The color development is stopped, and the intensity of the color is measured.

Background: FGF21 is a member of the FGF family and is the primary endogenous agonist for the FGF21 receptor. It is involved in various physiological processes and is associated with obesity. Studies have found that treatment with FGF21 analogues can normalize glucose utilization in obese mice and have shown some anti-obesity effects.

Synonyms: FGF-21; Fgf21

Applications: Bovine fibroblast growth factor 21, FGF21 ELISA kit (62.5-4000 pg/mL) is used to quantify FGF21 in serum, plasma, tissue homogenate samples of bovine, providing data to support a wide range of studies.

Research Area: Cytokine; Metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Tissue homogenates

Detection Range: 62.5-4000 pg/mL

Sensitivity: <15.6 pg/mL

Precision (Intra-assay): CV<8%

Precision (Inter-assay): CV<10%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out