AdipoUpX™ Human SREBF1 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human SREBF1 Lentiviral Particle (Overexpression) is professionally engineered for the long-lasting, stable overexpression of the human SREBF1 gene. SREBP-1c is a master regulator of lipogenesis; this stable integration tool allows for systematic investigation into fatty acid synthesis and glucose metabolism. The product is ready-to-use, high-titer, and all particles undergo comprehensive quality control to ensure safety and efficacy. Our vector system features a modular design, offering high flexibility for customization to meet specific metabolic modeling requirements.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: SREBF1

Full Name: Sterol regulatory element binding transcription factor 1

Gene Symbol: HMD; IFAP2; SREBP1; bHLHd1

Gene ID: 6720

RefSeq ID-1: NP_001005291.1

RefSeq ID-2: NM_001005291.3

Summary: SREBF1 acts as a master transcriptional regulator for de novo lipogenesis and sterol biosynthesis. Initially synthesized as an organelle-bound precursor, it moves to the nucleus after proteolytic cleavage to activate genes like the LDL receptor. Its SREBP-1c isoform is specifically induced by insulin, providing a direct molecular link between hyperinsulinemia and the increased hepatic fat synthesis observed in metabolic syndrome.

AdipoUpX™ Human SREBF1 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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