AdipoUpX™ Human SIRT1 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human SIRT1 MSCV Particle (Overexpression) is a powerful gene delivery tool designed to drive the stable expression of sirtuin 1, a key NAD⁺-dependent deacetylase. SIRT1 plays a pivotal role in regulating metabolic homeostasis, insulin sensitivity, and the response to caloric restriction. This ready-to-use particle is compatible with embryonic stem cells (ES), hematopoietic stem cells, and diverse mammalian cell types. The product supports flexible selection of reporter genes and is strictly screened through quality control protocols, including titer verification and sterility assays, to guarantee reliable performance.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: SIRT1

Full Name: Sirtuin 1

Gene Symbol: SIR2; SIR2L1; SIR2alpha

Gene ID: 23411

RefSeq ID-1: NP_001135970.1

RefSeq ID-2: NM_001142498.1

Summary: SIRT1 belongs to the sirtuin family of proteins and serves as an NAD-dependent deacetylase. As a human homolog of the yeast Sir2 protein, it acts as an intracellular metabolic sensor that links cellular energy states to gene expression. It is involved in epigenetic silencing and the regulation of metabolic adaptation, making it a critical factor in the study of longevity and metabolic stress.

AdipoUpX™ Human SIRT1 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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