AdipoUpX™ Human PHF21A Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human PHF21A Lentiviral Particle (Overexpression) is a high-titer system for the specific and stable expression of the human PHF21A gene. As a component of the BRAF-histone deacetylase complex, it is a key target for studying the epigenetic regulation of genes involved in energy balance and obesity. The lentivirus characteristic of genome integration establishes reliable cell models for longitudinal studies. Comprehensive quality control is performed to ensure product safety, and our platform supports the flexible customization of functional markers to meet diverse experimental needs.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: PHF21A

Full Name: PHD finger protein 21A

Gene Symbol: BHC80; NEDMS; BM-006; IDDBCS

Gene ID: 51317

RefSeq ID-1: NP_001095272.1

RefSeq ID-2: NM_001101802.3

Summary: PHF21A encodes BHC80, a component of the BRAF35/histone deacetylase (HDAC) repressor complex that suppresses neuron-specific gene expression through the repressor element-1 (RE1), also known as the neural restrictive silencer (NRS). While primarily studied in neurodevelopmental regulation, emerging evidence suggests that epigenetic repression mechanisms mediated by PHF21A may influence hypothalamic pathways controlling appetite, energy balance, and body weight. Altered chromatin regulation in neuronal circuits is increasingly recognized as a contributor to obesity susceptibility.

AdipoUpX™ Human PHF21A Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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