AdipoUpX™ Human HNF4A Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human HNF4A Lentiviral Particle (Overexpression) is a high-titer system for the specific and stable expression of the human HNF4A gene. As a master regulator of hepatic and pancreatic gene expression, its stable overexpression is essential for researching mature-onset diabetes and lipid transport regulation. The lentivirus characteristic of genome integration ensures the establishment of reliable cell models for metabolic signaling studies. Comprehensive quality control is performed on all viral particle products to ensure both product safety and the reproducibility of results.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: HNF4A

Full Name: Hepatocyte nuclear factor 4 alpha

Gene Symbol: TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; TCF-14; HNF4alpha

Gene ID: 3172

RefSeq ID-1: NP_000448.3

RefSeq ID-2: NM_000457.4

Summary: HNF4A encodes a nuclear transcription factor that binds DNA as a homodimer and regulates hepatic, intestinal, and pancreatic gene expression. It acts upstream of HNF1A and other genes involved in glucose and lipid metabolism. Mutations in HNF4A cause monogenic, autosomal dominant non-insulin-dependent diabetes and may contribute to metabolic disturbances that increase susceptibility to obesity. Alternative splicing generates multiple isoforms with potentially distinct regulatory roles.

AdipoUpX™ Human HNF4A Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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