AdipoUpX™ Human FOXO1 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human FOXO1 Lentiviral Particle (Overexpression) is a ready-to-use, high-titer vector particle designed for the efficient and stable overexpression of the human FOXO1 gene. This transcription factor is a central regulator of insulin signaling and hepatic gluconeogenesis; genome integration ensures the creation of stable cell lines for high-throughput metabolic screening. To ensure experimental safety and reproducibility, the product undergoes comprehensive quality control, including titer and mycoplasma testing, while offering flexible options for reporter gene selection.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: FOXO1

Full Name: Forkhead box O1

Gene Symbol: FKH1; FKHR; FOXO1A

Gene ID: 2308

RefSeq ID-1: NP_002006.2

RefSeq ID-2: NM_002015.3

Summary: FOXO1 encodes a forkhead transcription factor involved in gluconeogenesis, adipocyte differentiation, and oxidative stress response. It regulates energy homeostasis and fat storage, making it a key gene in obesity and insulin resistance pathways. Translocations with PAX3 are linked to certain cancers, but its metabolic role is critical in energy balance.

AdipoUpX™ Human FOXO1 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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