AdipoUpX™ Human CEBPA MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human CEBPA MSCV Particle (Overexpression) is engineered for efficient and stable expression of the human CEBPA gene. CEBPA is a master transcription factor regulating adipocyte differentiation, lipid storage, and metabolic gene programs central to obesity research. The MSCV retroviral backbone supports durable gene integration in stem cells, hematopoietic stem cells, and diverse mammalian models. Flexible reporter gene options are supported, and all viral particles are validated through rigorous QC screening, including titer, sterility, and mycoplasma assays.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: CEBPA

Full Name: CCAAT enhancer binding protein alpha

Gene Symbol: CEBP; C/EBP-alpha

Gene ID: 1050

RefSeq ID-1: NP_001272758.1

RefSeq ID-2: NM_001285829.2

Summary: The CEBPA gene produces a leucine zipper transcription factor that is a master regulator of body weight homeostasis and cell cycle control. By binding to CCAAT motifs in target promoters, it coordinates the differentiation of fat cells (adipocytes) and liver cells. Mutations in this gene are notably linked to acute myeloid leukemia, while its alternative translation into various isoforms allows it to fine-tune its regulatory impact on energy balance.

AdipoUpX™ Human CEBPA MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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