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AdipoUpX™ Human CARTPT Lentiviral Particle (Overexpression)

Cat. No.: V1225XX441
Species: Human
Target Gene: CARTPT
Vector System: Lentiviral
Modulation Type: Overexpression
AdipoUpX™ Human CARTPT Lentiviral Particle (Overexpression)
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Product Overview

Description: AdipoUpX™ Human CARTPT Lentiviral Particle (Overexpression) is a ready-to-use, high-titer vector particle professionally engineered for the stable overexpression of the human CARTPT gene. By integrating into the host cell genome, it allows for the establishment of reliable cell models to investigate CART’s role as a potent anorexigenic neuropeptide. To ensure experimental reproducibility and safety, every batch undergoes comprehensive quality control, including titer, sterility, and mycoplasma testing. Our modular system provides high flexibility for the customization of cell-specific promoters and fluorescent markers.
Production Cell Line: HEK293T
Promoter: Ubi; CMV; EF1a; Others
Product Availability: Produced Upon Order

Specification

Titer Test: qPCR
Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.
Sterility Test: This product was certified sterile following successful microbial growth analysis.
Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.
Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.
Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.
Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.
Shipping Condition: Our lentivirus viral products are shipped using dry ice.
Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.
Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.
Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: CARTPT
Full Name: CART prepropeptide
Gene Symbol: CART
Gene ID: 9607
RefSeq ID-1: NP_004282.1
RefSeq ID-2: NM_004291.4
Summary: CARTPT encodes a precursor protein that undergoes proteolytic processing to yield several biologically active peptides, collectively known as CART peptides. These peptides are essential modulators in the central nervous system, playing a critical role in appetite regulation, energy balance, the maintenance of body weight, reward, addiction, and the stress response. Its role was first highlighted in rodents, where its transcript expression was upregulated following cocaine and amphetamine administration. Due to its central influence over satiety and energy expenditure, mutations in CARTPT are associated with susceptibility to obesity in humans.

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