AdipoUpX™ Human BBS5 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human BBS5 Lentiviral Particle (Overexpression) is a powerful gene delivery tool specifically designed for the stable overexpression of the human BBS5 gene. This protein’s ability to bind phosphoinositides is vital for ciliary membrane recruitment; genome integration allows for the establishment of stable cell lines to study these complex trafficking mechanisms. All particles undergo rigorous quality control screening to ensure experimental reliability. Our customizable platform supports the flexible inclusion of fluorescent tags for real-time visualization of protein localization.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: BBS5

Full Name: Bardet-Biedl syndrome 5

Gene ID: 129880

RefSeq ID-1: NP_689597.1

RefSeq ID-2: NM_152384.3

Summary: BBS5 is directly associated with Bardet-Biedl syndrome (BBS), a severe autosomal recessive ciliopathy. The BBS5 protein is a crucial component of the BBSome complex and is required for the proper structure and function of the primary cilium. Experimental data confirm that BBS5 is expressed in ciliated cells and is necessary for ciliary formation. Mutations in BBS5 result in the BBS phenotype, whose primary features include retinal dystrophy, polydactyly, renal abnormalities, learning disabilities, and severe, early-onset obesity, underscoring the link between ciliary transport and metabolic control.

AdipoUpX™ Human BBS5 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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