AdipoUpX™ Human BBS2 MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human BBS2 MMLV Particle (Overexpression) is a stable integration tool for the specific and efficient overexpression of the human BBS2 gene. This product allows for the systematic investigation of BBSome assembly and its influence on leptin receptor signaling within the cilia. The MMLV vector provides long-lasting overexpression, making it a reliable asset for establishing permanent cell models. Product quality is ensured by rigorous purification, titer validation, and comprehensive QC screening to guarantee safe and high-fidelity experimental results.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: BBS2

Full Name: Bardet-Biedl syndrome 2

Gene Symbol: BBS; RP74

Gene ID: 583

RefSeq ID-1: NP_001364385.1

RefSeq ID-2: NM_001377456.1

Summary: BBS2 is a member of the Bardet-Biedl syndrome (BBS) gene family. The proteins encoded by this family are functionally diverse but share a common critical role in cilia formation and function. BBS2 localizes to the basal bodies and ciliary axonemes and is one of the eight proteins that assemble to form the BBSome complex. This complex is essential for intracellular trafficking via microtubule-related transport to and from the cilium. Mutations in BBS2 cause BBS, a ciliopathy defined by a pleiotropic phenotype including severe pigmentary retinopathy, obesity, polydactyly, renal malformation, and cognitive disability.

AdipoUpX™ Human BBS2 MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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