AdipoUpX™ Human HNF4A MMLV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human HNF4A MMLV Particle (Overexpression) is a high-titer MMLV particle designed for the efficient and stable overexpression of the human HNF4A gene. This nuclear receptor is critical for hepatic lipid metabolism and the regulation of insulin secretion. The MMLV system’s stable integration into dividing host cells makes it an ideal tool for investigating the genetic drivers of Maturity-Onset Diabetes of the Young (MODY). Every viral batch is verified through comprehensive quality control screening (titer, sterility, and mycoplasma) to ensure the highest efficacy.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Complete verification of the vector genome's integrity was achieved for every viral preparation using the PCR method.

Sterility Test: The final viral preparation was confirmed sterile after testing.

Mycoplasma Test: Mycoplasma contamination testing met all release specifications for all product lots.

Other QC: We also provide additional testing services tailored to specific project requirements. Additionally, we conduct customized assessments to analyze the expression levels and biological functions of genetically modified organisms.

Storage: The product must be immediately transferred to -80 °C storage upon its arrival.

Stability: For long-term storage, the product guarantees excellent stability for 6-12 months at -80°C. After thawing, refrigeration at 4°C allows use of the working solution for 2-3 weeks.

Shipping Condition: Our MMLV retroviral products are shipped using dry ice.

Handling Notes: Due to their high sensitivity to temperature fluctuations and damaging freeze-thaw cycles, viral particles should be aliquoted into low-protein-binding tubes immediately upon receipt to maintain titer and product integrity. This procedure must be conducted within a biosafety cabinet.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: All products have undergone rigorous internal QC inspections as part of our company's commitment to product quality. However, due to the diversity and complexity of experimental conditions, users bear ultimate responsibility for product storage, handling, and compliance with all safety and biosafety protocols, and we offer no guarantee of product performance or experimental results in any specific application.

Target Profile

Gene Name: HNF4A

Full Name: Hepatocyte nuclear factor 4 alpha

Gene Symbol: TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; TCF-14; HNF4alpha

Gene ID: 3172

RefSeq ID-1: NP_000448.3

RefSeq ID-2: NM_000457.4

Summary: HNF4A is a nuclear transcription factor that functions as a homodimer to control a complex network of genes in the liver, gut, and kidneys. It regulates the expression of other key factors, such as HNF1-alpha, creating a hierarchy of metabolic control. Mutations in this gene cause a specific form of monogenic diabetes known as MODY1 (Maturity-Onset Diabetes of the Young), where the body fails to properly regulate glucose due to impaired insulin production and hepatic gene expression.

AdipoUpX™ Human HNF4A MMLV Particle (Overexpression)

Product Overview

Specification

Target Profile

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