AdipoUpX™ Human BBS10 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human BBS10 Lentiviral Particle (Overexpression) is a ready-to-use, high-titer vector particle designed for the stable overexpression of the human BBS10 gene, a chaperonin critical for BBSome assembly. Its stable expression facilitates in-depth research into the early stages of ciliogenesis and its role in severe obesity. To ensure experimental reproducibility, every batch undergoes rigorous quality control, including titer and sterility testing. Our platform features a modular design, providing researchers the ability to flexibly select promoters for targeted expression.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: BBS10

Full Name: Bardet-Biedl syndrome 10

Gene Symbol: C12orf58

Gene ID: 79738

RefSeq ID-1: NP_078961.3

RefSeq ID-2: NM_024685.3

Summary: BBS10 is another member of the BBS gene family, associated with Bardet-Biedl syndrome type 10, which presents with progressive retinal degeneration, obesity, polydactyly, renal anomalies, and cognitive disability. Unlike most BBS proteins, BBS10 likely functions as a molecular chaperone, with distant homology to type II chaperonins, influencing the folding and stability of ciliary or basal body proteins. Disruption of BBS10 expression impairs ciliogenesis in preadipocytes.

AdipoUpX™ Human BBS10 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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