AdipoUpX™ Human ATF6 MSCV Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ATF6 MSCV Particle (Overexpression) is a stable retroviral gene delivery system engineered for sustained overexpression of the human ATF6 gene. ATF6 is a central regulator of endoplasmic reticulum stress responses, which are strongly implicated in obesity, insulin resistance, and adipocyte dysfunction. The MSCV platform supports long-term gene integration in stem cells, hematopoietic stem cells, and other mammalian cell lines relevant to metabolic research. Each viral preparation is subjected to validated titer determination, sterility testing, and mycoplasma screening to ensure reproducibility and experimental safety.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: Final QC confirmed the correct molecular size and integrity of the insert through specific PCR amplification.

Sterility Test: No microbial contamination was detected in any product.

Mycoplasma Test: No mycoplasma presence was detected in this final viral preparation upon comprehensive testing.

Other QC: Our service scope includes both customized supplementary testing and comprehensive in vitro/in vivo transduction assessments.

Storage: The necessity of immediate transfer to -80 °C storage following receipt is crucial for upholding the product's integrity.

Stability: Biological activity remains intact for 6-12 months when stored at -80°C. Once removed from the freezer, the diluted working material is stable for up to 2-3 weeks at 4°C.

Shipping Condition: Our MSCV retroviral products are shipped using dry ice.

Handling Notes: Store the vector according to specified conditions and aliquot it immediately upon receipt into low-protein-binding tubes, which is necessary to maintain titer because viral particles are highly sensitive to both temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Throughout all operational processes, users bear ultimate responsibility for product storage, handling, and compliance with all safety protocols, laws, regulations, and biosafety requirements. Although our company commits to product quality via rigorous internal QC inspections, the complexity of experimental conditions means we cannot guarantee product performance or experimental results in any specific application.

Target Profile

Gene Name: ATF6

Full Name: Activating transcription factor 6

Gene Symbol: ACHM7; ATF6A; ATP6alpha

Gene ID: 22926

RefSeq ID-1: NP_031374.2

RefSeq ID-2: NM_007348.4

Summary: ATF6 encodes an endoplasmic reticulum–resident transmembrane transcription factor that functions as a key sensor and mediator of the unfolded protein response (UPR). Upon ER stress, ATF6 undergoes proteolytic cleavage, releasing an active fragment that translocates to the nucleus and induces expression of ER chaperones and stress-response genes. Dysregulation of ATF6 signaling has been linked to altered lipid metabolism, insulin resistance, and metabolic stress, implicating this pathway in obesity-associated disorders.

AdipoUpX™ Human ATF6 MSCV Particle (Overexpression)

Product Overview

Specification

Target Profile

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