AdipoUpX™ Human ACOT11 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ACOT11 Lentiviral Particle (Overexpression) is professionally engineered for the long-lasting, stable overexpression of the human ACOT11 gene. This acyl-CoA thioesterase is a master regulator of brown adipose tissue thermogenesis and energy efficiency. The product is ready-to-use and high-titer, with every lot passing rigorous quality control for titer, sterility, and purity. Our modular design provides researchers with the ability to flexibly select markers for high-fidelity tracking of fatty acid utilization.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: ACOT11

Full Name: Acyl-CoA thioesterase 11

Gene Symbol: BFIT; THEA; THEM1; STARD14

Gene ID: 26027

RefSeq ID-1: NP_056362.1

RefSeq ID-2: NM_015547.4

Summary: ACOT11 encodes an acyl-CoA thioesterase that hydrolyzes fatty acyl-CoA esters into free fatty acids and coenzyme A. In mouse models, ACOT11 expression in brown adipose tissue is induced by cold exposure and suppressed by warmth, indicating a role in thermogenesis. Notably, higher expression of ACOT11 has been observed in obesity-resistant mice compared with obesity-prone strains, suggesting a protective role against excessive fat accumulation. Alternative splicing generates multiple transcript variants.

AdipoUpX™ Human ACOT11 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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