AdipoUpX™ Human ACAT2 Lentiviral Particle (Overexpression)

Product Overview

Description: AdipoUpX™ Human ACAT2 Lentiviral Particle (Overexpression) is a ready-to-use, high-titer vector particle professionally engineered for the stable overexpression of the human ACAT2 gene. By facilitating the integration of the target gene into the host cell genome, it serves as a reliable system for researching intracellular cholesterol esterification and its impact on lipid droplet formation. Every batch undergoes rigorous quality control, including titer, sterility, and mycoplasma testing, ensuring experimental reproducibility and safety. Our vector platform supports the flexible selection of reporter genes for precise tracking.

Production Cell Line: HEK293T

Promoter: Ubi; CMV; EF1a; Others

Product Availability: Produced Upon Order

Specification

Titer Test: qPCR

Insert Verification: PCR analysis was performed to verify the complete genomic sequence of all viral vector preparations.

Sterility Test: This product was certified sterile following successful microbial growth analysis.

Mycoplasma Test: The production quality control confirmed the absence of any mycoplasmal agents.

Other QC: This comprehensive process includes customized supplementary testing alongside in vitro and in vivo transduction assessments to elucidate transgene expression and functionality.

Storage: To maintain product integrity, immediate transfer of the vector to a -80 °C is essential following its receipt.

Stability: Continuous storage at -80°C ensures excellent long-term stability for 6-12 months. Once thawed, the product's working solution should be refrigerated at 4°C and used within 2-3 weeks.

Shipping Condition: Our lentivirus viral products are shipped using dry ice.

Handling Notes: Ensure all operations are performed within a biosafety cabinet; upon receiving the vector, immediately aliquot it into low-protein-binding tubes and store according to specified conditions, thus avoiding the titer loss caused by sensitivity to temperature fluctuations and freeze-thaw cycles.

Intended Use: This product is intended for research use only and is not for use in diagnosis or therapeutic applications.

Product Disclaimer: Given the diversity and complexity of experimental conditions, we do not guarantee product performance or experimental results in any specific application, despite our company's commitment to product quality and the rigorous internal QC inspections all products have undergone. Ultimate responsibility remains with the user for all operational processes, including storage, handling, and compliance with safety and biosafety protocols.

Target Profile

Gene Name: ACAT2

Full Name: Acetyl-CoA acetyltransferase 2

Gene ID: 39

RefSeq ID-1: NP_001290182.1

RefSeq ID-2: NM_001303253.1

Summary: ACAT2 encodes cytosolic acetoacetyl-CoA thiolase, an enzyme critical for lipid metabolism, including cholesterol and fatty acid synthesis. Variations in this gene may influence lipid storage and fat accumulation, contributing to obesity risk. In both humans and mice, ACAT2 partially overlaps with the 3′ region of TCP1 on opposite DNA strands. Alternative splicing produces multiple isoforms.

AdipoUpX™ Human ACAT2 Lentiviral Particle (Overexpression)

Product Overview

Specification

Target Profile

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