Sheep growth hormone (GH) ELISA kit

Cat. No.: 0126WXX-131

Assay Type: Quantitative ELISA

Target Species: Sheep

Assay Target: GH

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INQUIRY Datasheet MSDS

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Product Overview

Description: Sheep growth hormone (GH) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of GH in sheep samples with a minimum detectable dose (sensitivity) of 0.1 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This GH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate is pre-coated with a monoclonal antibody specific for GH. Standards or samples are added, followed by an HRP-conjugated polyclonal antibody to form a "sandwich" complex. After incubation and thorough washing, substrate solutions are added for color development. The intensity is directly proportional to the GH concentration. By plotting a standard curve of O.D. versus concentration, the GH level in each sample is interpolated.

Background: Growth hormone (GH) is a crucial endocrine hormone that plays a key role in growth regulation and metabolic control. It primarily promotes bodily growth by stimulating the liver and other tissues to secrete insulin-like growth factor-1 (IGF-1), while also enhancing amino acid uptake and protein synthesis. Deficiencies in GH can lead to growth hormone deficiency disorders, such as dwarfism. Research indicates that GH induces lipolysis through direct lipolytic effects in adipose tissue and influences obesity to a certain extent.

Synonyms: GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary growth hormone; Growth hormone, normal

Applications: Sheep growth hormone (GH) ELISA kit is used to quantify GH in serum, plasma, cell culture supernatants, body fluid, and tissue homogenate of sheep, providing data to support research in a wide range of areas, including developmental biology, obesity, endocrinology, neuro science, metabolism, and others.

Research Area: Developmental biology; Obesity; Endocrinology; Neuro science; Metabolism

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Recovery: 94-103%

Storage: Store at 2-8°C.

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