Human apolipoprotein AI (Apo-AI) ELISA kit

Cat. No.: OB0625WXX-114

Assay Type: Quantitative competitive ELISA

Target Species: Human

Assay Target: Apo-AI

Size:

Human apolipoprotein AI (Apo-AI) ELISA kit

INQUIRY Datasheet MSDS

Add to basket

Product Overview

Description: Human apolipoprotein AI (Apo-AI) ELISA kit is an ELISA-based in vitro research tool designed specifically for the quantitative detection of Apo-AI in human with a specificity of 0.1 ng/mL.

Assay Principle: The assay samples and buffer are incubated with Apo-AI-HRP conjugate in a pre-coated plate for one hour. At the end of the incubation period, the wells are decanted and washed five times. The wells are then incubated with HRP enzyme substrate. The products of the enzyme-substrate reaction form a blue color complex. Finally, the reaction is stopped by adding a stop solution, which turns the solution yellow. The color intensity is measured spectrophotometrically at 450 nm on a microplate reader. The color intensity is inversely proportional to the concentration of Apo-AI because Apo-AI in the sample competes with the Apo-AI-HRP conjugate for the anti-Apo-AI antibody binding site. Since there are a limited number of binding sites, as more sites are occupied by Apo-AI in the sample, fewer sites can bind to the Apo-AI-HRP conjugate. A standard curve is plotted between color intensity (O.D.) and standard concentration. The concentration of Apo-AI in each sample is derived from this standard curve.

Background: Apo-AI is the major protein component of high-density lipoprotein (HDL) in plasma and is involved in the transport of cholesterol from tissues to the liver for excretion.

Synonyms: APOA1, Apolipoprotein A1, Apolipoprotein A-I, HPALP2

Applications: Human apolipoprotein AI (Apo-AI) ELISA kit is an important tool for the quantitative detection of Apo-AI in various biological samples (serum, plasma, cell culture supernatants, body fluid, tissue homogenate) of humans.

Research Area: Metabolism; Obesity research

Specification

Sample Type: Serum; Plasma; Cell culture supernatants; Body fluid; Tissue homogenate

Sensitivity: 0.1 ng/mL

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

Related Products

Inquiry

Inquiry Basket

Delete selected Check Out