Rat neurotrophic tyrosine kinase receptor type 2 (NTRK2) ELISA kit (0.156-10 ng/mL)

Cat. No.: 0126WXX-1183

Assay Type: Quantitative sandwich ELISA

Target Species: Rat

Assay Target: NTRK2

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Rat neurotrophic tyrosine kinase receptor type 2 (NTRK2) ELISA kit (0.156-10 ng/mL)

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Product Overview

Description: Rat neurotrophic tyrosine kinase receptor type 2 (NTRK2) ELISA kit (0.156-10 ng/mL) is an ELISA-based in vitro research tool designed specifically for the quantitative detection of NTRK2 in rat samples with a range of 0.156-10 ng/mL and a minimum detectable dose (sensitivity) of 0.053 ng/mL. The kit is highly sensitive and easy to use.

Assay Principle: This kit employs the sandwich enzyme immunoassay technique. A microtiter plate has been pre-coated with an antibody specific to NTRK2. Standards or samples are added to the wells, followed by a biotin-conjugated antibody specific to NTRK2. After incubation and washing, avidin conjugated to HRP is added to each well. After a second incubation and wash, a TMB substrate solution is added. Only wells containing NTRK2, the biotinylated antibody, and enzyme-conjugated avidin will exhibit a color change. The reaction is terminated by the addition of a sulphuric acid stop solution, and the color intensity is measured spectrophotometrically at 450nm ± 10nm. The concentration of NTRK2 in the samples is determined by comparing the O.D. of the samples to the standard curve.

Background: NTRK2, also known as TrkB, is a high-affinity receptor for brain-derived neurotrophic factor (BDNF). The BDNF/TrkB signaling pathway is crucial for neuronal survival, differentiation, and plasticity in the central nervous system (CNS). The link between NTRK2 and obesity research primarily manifests in the CNS regulation of appetite: BDNF serves as a key anorexigenic (appetite-suppressing) signal. Defects in NTRK2 expression and function in the hypothalamus weaken BDNF signaling, leading to overeating, reduced energy expenditure, and severe obesity. Consequently, NTRK2 serves as a key signaling integration point in central obesity regulation pathways (acting synergistically with the MC pathway, leptin signaling, etc.).

Synonyms: OBHD; TRKB; DEE58; trk-B; EIEE58; GP145-TrkB; EC 2.7.10.1; Neurotrophic tyrosine kinase, receptor, type 2; BDNF-Tropomyosine receptor kinase B

EC NO.: 2.7.10.1

Applications: Rat neurotrophic tyrosine kinase receptor type 2 (NTRK2) ELISA kit (0.156-10 ng/mL) is used to quantify NTRK2 in tissue homogenates, cell lysates, and other biological fluids of rat, providing data to support research in a wide range of areas, including signal transduction, neurodevelopment, obesity, etc.

Research Area: Signal transduction; Neurodevelopment; Obesity

Specification

Sample Type: Tissue homogenates; Cell lysates; Other biological fluids

Detection Range: 0.156-10 ng/mL

Sensitivity: 0.053 ng/mL

Precision (Intra-assay): CV<10%

Precision (Inter-assay): CV<12%

Cross-reactivity: No significant cross-reactivity or interference was observed.

Storage: Store at 2-8°C.

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